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Generation of Squalene Synthase Mutants
Using Classical Methods


Squalene Synthase Mutants


Yeast normally cannot take up exogenous sterols unless grown under anaerobic conditions. The ergosterol-dependent mutants isolated as described above have been shown to carry a mutation not only in the ERG9 gene (coding for squalene synthase), but also in an uncharacterized gene (or genes) that confer the ability to take up sterols under aerobic conditions. We refer to this sterol uptake mutation as the sue mutation (Sterol Uptake Enhancement).