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Table 1
Expression of Different Recombinant Glucosamine
Synthase Genes in E. coli

Recombinant Glucosamine Genes in <i>E. coli</i>

Bacterial glmS and yeast GFA1 gene coding sequences were inserted into a pET vector and transformed into an E. coli strain (genotype: Δnag, manXYZ, DE3). Strains containing a free-replicating plasmid or an integrated expression cassette were grown in shake flasks under IPTG-induced conditions at 30°C for 26 hours in a defined mineral salt medium (M9A) supplemented with glucose.