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Table 2
GlcNAc Production by Over-Expressing Glucosamine Synthase and Glucosamine-N-Acetyltransferase (GNA1)

GlcNAc Production Table

Three GNA1 coding sequences from yeast and a higher plant were inserted into pET24d(+), forming T7lac-GNA1 expression cassettes. Recombinant plasmids were transformed into E. coli strain 7107-18, which contained an integrated expression cassette of T7lac-glmS*54. Resulting strains with GNA1 expression vectors were grown for 23 hours in a simple mineral medium (M9B) supplemented with 40 g L-1 glucose, 10 g L-1 ribose, and 5 g L-1 yeast extract. Cultures were induced with 0.2 mM IPTG at the time of inoculation.