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Table 4
In Vitro Synthesis of GlcN and GlcNAc

<i>In Vitro</i> Synthesis Table

Crude enzyme extracts were prepared from IPTG-induced cultures and incubated with NH4Cl (200 mM) and glutamine (20 mM) as amino donors. Amounts of amino sugars formed were determined by a colorimetric assay using Elson-Morgan reagents. Data confirmed that the NagB enzyme uses only ammonia as the amino donor in GlcN-6-P formation.