Protein Expression in Bacillus subtilis
Bacillus subtilis grows as fast as E. coli to high cell density in simple minimal media. Unlike E. coli, however, this Gram positive bacterium is nonpathogenic, nontoxigenic and it produces no endotoxin. Another attribute of B. subtilis is its nature capacity to secrete active enzymes at high levels. Historically, this microorganism is the most widely used production host of enzymes for feed and food applications. Carbohydrase and protease produced from B. subtilis have been granted GRAS (Generally Recognized as Safe) status by U.S. FDA. Multiple inducible or constitutive promoters and various secretion signal peptides are available for optimization of protein production. Strains with multiple protease genes deleted have been developed to minimize proteolytic degradation of heterologous protein. BTR Bacillus expression system offers unique features and advantages comparing to other common systems. Please inquire for more details.
Information on protein expression in other hosts can be found in PowerPoint slideshows BTR multiple host systems for protein expression (pdf) and Protein expression examples (pdf).